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Among the last years, environmental concerns have raised the interest for biofuels. Ethanol, blended with gasoline seems particularly suited for the operation of internal combustion engines, and has been in use for severals years in some countries. However, it has a strong impact on engine performance, which is emphasized on recent engine architectures, with downsizing through turbocharging and variable valve actuation. Taking all the benefits of ethanol-blended fuel thus requires an adaptation of the engine management system. This paper intends to assess the effect of gasoline-ethanol blending from this point of view, then to describe a mean-value model of a fuel-flexible turbocharged PFI-SI engine, which will serve as a basis for the development of control algorithms. The focus will be in this paper on ethanol content estimation in the blend, supported by both simulation and experimental results.