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In-cylinder velocity measurements were acquired in a two-valve, single-cylinder research engine to study the bulk fluid motion and small-scale turbulence. Different port geometries (two), different port orientations (two) and both shrouded and non-shrouded intake valves were tested to vary the intake-generated flow. Tests were performed at engine speeds of 300, 600, 900 and 1200 RPM with an atmospheric intake pressure. Prior to testing on the engine, the different head configurations were tested on a steady flow bench. Particle image velocimetry data were taken on a single plane, parallel to the piston surface, in the engine using both a low magnification to characterize the large-scale flow phenomena, and a high magnification to characterize the turbulence field. The low-magnification results showed that the swirl center location was relatively insensitive to engine speed, but did change position throughout the cycle.