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A burning process in a combustion chamber of an internal combustion engine is very important to know the maximum temperature of the gases, the speed of combustion, the ignition delay time of fuel and air mixture exact moment at which ignition will occur. The automobilist industry has invested considerable amounts of resources in numerical modeling and simulations in order to obtain relevant information about the processes in the combustion chamber and then extract the maximum engine performance control the emission of pollutants and formulate new fuels. This study aimed to general construction and instrumentation of a shock tube for measuring shock wave. As specific objective was determined reaction rate and ignition delay time of diesel, biodiesel and ethanol doped with different levels of additive enhancer cetane number. The results are compared with the ignition delay times measured for other authors.