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The increasing fuel demand, decreasing natural reserves and environmental consciousness have together led to the testing and implementation of new fuels and blending components for internal combustion engines. Biofuels are very commonly added to fossil fuels, most commonly ethanol to gasoline and FAME to diesel. Harmonizing their properties with the engines is a great challenge for the automotive and oil industry. The increasing demand for diesel and decreasing demand for gasoline in Europe raised the question of how it is possible to increase the amount of bio extenders in diesel. Butanol as a potential second generation biofuel could be a future option for blending with diesel. A comprehensive analytical and empirical testing sequence was carried out in the MOL Group's Product Development laboratories to check the application properties of butanol-diesel mixtures.